Fig 1: Effect of dietary RES on mitochondrial autophagy in the liver of gibel carp at 6 h and 12 h after LPS injection. (A) Ultrastructural changes of hepatocytes under TEM. Scale bar = 500 nm. Red arrows represent the autophagosome. (B) Mitophagy-related mRNA levels were determined with qRT-PCR. (C) Western blots for P62 and LC3B and quantification results. (D) Co-localization analysis of P62 (green) and LC3 (red) in the hepatocytes of gibel carp and the relative fluorescence intensity. DAPI staining is shown in blue. Bars = 50 µm. Data are mean ± SEM (n = 6). The lowercase letters represent significant differences among diverse LPS durations in the CON group (P < 0.05); the uppercase letters represent significant differences among diverse LPS durations in the RES group (P < 0.05). *(P < 0.05) and **(P < 0.01) represent significant differences between the CON and RES groups. Parkin, parkin RBR E3 ubiquitin-protein ligase; pink1, PTEN-induced kinase 1; optn, optineurin; bnip3, BCL2 interacting protein 3; lc3b, microtubule-associated proteins 1A/1B light chain 3B; p62, P62.